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Software

Simply, efficiently and quickly analyze multicolor, multiparametric data from flow cytometers of today and tomorrow.

Kaluza for Gallios Acquisition Software

Analytical muscle for your collected data

With four possible laser configurations scalable to 10 colors, sophisticated forward and side scatter detectors, state-of-the-art optics, and other innovative features, Gallios delivers powerful, versatile performance. And Kaluza provides the analytical muscle to make the most of the data generated.

Download our free offline software which allows users to author Kaluza protocols and worklists for the Gallios flow cytometer on their own computers.

Features

Easy to learn and easy to use at all experience levels


Kaluza for Gallios is built on the strong, innovative foundation of Kaluza Analysis Software. With Kaluza you can power through data sets containing multiple colors, plots and events with speed and precision. The result: your flow cytometry research can find that extra gear to get you to your objective sooner.
 

Kaluza Means Ease

  • No database to impact performance—save your files to where you specify
  • Simple user interface—easy to learn and quick to use
  • Reconfigure your workspace with auto-layout feature for easy plot creation and sizing, zoom in/out, and guided drag and drop
  • Visual management tools help you understand what is happening to your experiment and cytometer
  • Make a mistake? Undo it, with unlimited undos!
  • Set voltages and compensation while in Setup Mode then acquire data
  • Provide as much or as little sample information as you like before running an experiment
  • No user profiles or login required to run acquisition
 

Flow Cytometry,fcs files,flo jo,flojo,plot types,multi-parametric,multi parametric,multi color,multi-color,real time analysis,authoring software,real time analysis


Kaluza Means Efficiency

 
  • Will exhibit eight times higher event limit than existing Gallios software
  • Integrate Kaluza into your existing lab tools with standard FCS 3.1 files
  • Use offline software to create protocols and worklists to make your instrument time more productive
  • Use Gallios Simulator to train on the use of the instrument so you don’t have to run a live experiment every time you train
  • Cleanse on Close means that the cytometer will be ready for action the next morning
  • Already have a Gallios? No problem—Kaluza Acquisition will read Gallios protocols
     

Flow Cytometry,fcs files,flo jo,flojo,plot types,multi-parametric,multi parametric,multi color,multi-color,real time analysis,authoring software,real time analysis


Kaluza Means Speed

 
  • Set up a 10 color experiment in seconds
  • Change voltages and compensation quickly, accurately and intuitively
  • Create compensation matricies with ease using compensation wizard
  • Create gates and access editing tools with innovative radial menu that keeps mouse clicks to a minimum
  • Easily load files into Kaluza Analysis Software for more in-depth analysis
  • Flexible carousel control allows you to configure, load and walk away. Create a protocol with one click using the “Add All Plots” feature
 

Flow Cytometry,fcs files,flo jo,flojo,plot types,multi-parametric,multi parametric,multi color,multi-color,real time analysis,authoring software,real time analysis

User Interface

Innovative Radial Menu 


Kaluza´s novel radial menu selection is invoked by a simple right mouse click. It brings all of the software functions right to where you are working on the screen and removes the need to repeatedly journey to the menu bar at the top of the screen. This saves the time spent moving the cursor, hunting for menu items as well as a dramatically reducing the multiple mouse clicks needed to perform simple functions on other software platforms.

Menu Details
On-plot access to: 
  • Creating gates
  • Formatting
  • Annotating
  • Statistics


Composite Analysis 


Challenges of Flow Analysis Today
Today, more colors requires more plots, events, protocol complexity and ultimately increased analysis time. Kaluza makes use of accelerated computation technologies to reduce your analysis time.

Load Multiple files directly from Explorer
Easily compare datasets with a composite analysis in a single workspace 

Seamless Integration
Easily reanalyze .fcs files from the FC 500, Navios™, Gallios™ including protocol and gates.
 

Computer Specs

Selecting a Computer for Kaluza 1.3 


The Kaluza software requires a Microsoft Windows PC. If you have one now, it´s likely that Kaluza will run on it. However, Kaluza offers the ability to dynamically update gating and statistics in real-time. This kind of analysis can be very demanding and you may find that you´re not happy with the way your current system handles it. While you can dial back this feature so that it works on your system, you may want to take this opportunity to upgrade to a better one. This guide helps you ensure that whatever you pick will meet your needs when you use Kaluza. What everyone calls a computer is really a computer system, and the pieces that make up this system determine how well it will work with Kaluza. It´s easiest to break this down into the main components.

Display:

The display subsystem has a really big impact on your Kaluza experience. At a minimum, you need a monitor that can handle 1024×768 with a normal display adapter. However, Kaluza has been optimized for high-resolution wide-screen displays. If you´re getting a new desktop system it is recommended that you get a 24-inch wide-screen monitor capable of 1920×1200 resolution. If you are working with enough plots, a 30-inch 2560×1600 display is very nice. However, it does cost a lot more and take up quite a bit of space. You don´t need a high-end display adapter, but you may want to get an NVidia brand one, depending on your other choices. 

Hard Disk:

Kaluza doesn´t have demanding hard disk requirements. Version 1.3 only takes up a little more than 20MB of space, and there´s no routine disk access required. However, depending on the files you are working with you may want to make an additional investment here. If you´re working with a few 1MB listmode files, then the disk system won´t make much difference. However, if the files you´re using are tens or even hundreds of megabytes, you might want to get something faster. A good test is to take some files that you routinely use and copy them. If Windows can´t copy them quickly, then Kaluza can´t do any better. Some higher end options include high spindle speeds (10K RPM) and large on-drive caches. RAID configurations can also give you additional speed and data integrity. The bottom line here is that Kaluza doesn´t set the disk requirements; your data does. If your data is big enough that you struggle with it outside of Kaluza, then Kaluza will probably be slow to load it.

Network:

Kaluza doesn´t have network requirements, but just as with the hard disk requirements, you want to consider your data and what you do with it. If you´re going to keep your data on a network drive, then you should have the fastest connection you can manage.

Operating System:

Kaluza requires Windows XP 32-bit SP3, Windows Vista 32-bit SP2, Windows 7/32 bit and Windows 7/64 bit.

RAM:

Kaluza likes RAM. With a 32-bit operating system you can´t take advantage of more than 3 or 4 gigabytes, but it´s very nice to have that much. Exactly how much you need is determined by what you´re doing. If you only work with a few 10K event files at a time, you can probably get by with less than a gigabyte. If you are working with really big files, then you´re going to want more. Generally 2GB is a good starting point. However, RAM is pretty cheap and if you have the choice you should probably get 3 or 4. 

CPU:

CPUs have gotten fast enough that for many tasks they´re almost too fast. If a 2GHz CPU recalculates your spreadsheet instantly, then do you really need a 3GHz one? If you´re using Kaluza, especially with lots of events and the dynamic update feature on, then the answer is yes. Lots of things determine how fast a CPU is, but you only usually have control over brand/model, clock speed, and number of cores/CPU count. (A core is the heart of a CPU. You can have more than one on a physical chip, which will make your computer faster if the software you´re using is written the right way.)

For Kaluza, the brand and model isn´t much of a deciding factor. Certain ones benchmark faster than others, and the faster ones are better for Kaluza, but it will work with all of them. The most important things for Kaluza are the number of cores and the speed of those cores. There´s no hard and fast rule, Kaluza will use whatever you give it, but this table gives some general guidance:
 
Events Cores
10,000 Single
100,000 Dual
1,000,000 Quad
10,000,000 Dual with Tesla card

The bottom line here is that for files with only 10k events or so, just about anything is acceptable. As you get into the hundred-thousand event range you need to be providing more power. When you get up to a million events, it takes some serious hardware to recalculate everything in real time. Of course, you can always turn down the dynamic update, but why buy a new system if you´re going to do that?

Tesla:

Throughout this guide, reference has been made to a special configuration, and the Tesla is it. The Tesla is a supercomputer coprocessor card from NVidia called the Tesla C1060. If Kaluza finds one when it starts, it will use it for all of its calculations. This card has 240 CPUs, 4GB of RAM, and is the ultimate way to process large amounts of data with Kaluza. If you´re using this card, you don´t need extra CPU cores or extra RAM because Kaluza uses what´s on the Tesla card. Be warned that the Tesla card has some unique power requirements. If you want to use one, it is best to build a system around it rather than try to add one to an existing system. It´s usually best to start with a vendor´s workstation class system if you want a Tesla Kaluza workstation.

Kaluza FAQ

What is Kaluza?

Kaluza Software is a revolutionary flow cytometry analysis software solution designed for high-content data. With features designed to make your life easier in the lab, Kaluza aims to provide a greater understanding of the data generated by modern cytometers.

 

Is Kaluza compatible with the FCS files from my system?

Kaluza is compatible with files that comply with the FCS standard through the most recently released standard. The software has been tested against a large repository of these files, but there are also tools are available within Kaluza to ensure that parameters are loaded properly (i.e., linear vs log).

 

Where can I purchase the Kaluza software?

Kaluza software can be purchased by selecting the Request a Quote tab below.    

 

Where did Kaluza get its name?

Theodor Franz Eduard Kaluza (November 9, 1885 - January 19, 1954) was a German mathematician and physicist known for the Kaluza-Klein theory involving field equations in five-dimensional space. His idea that fundamental forces can be unified by introducing additional dimensions re-emerged much later in string theory. His theories attempted to rationalize complex multidimensional space which is analogous to the challenges faced with when analyzing complex flow cytometry data.

 

How is Kaluza licensed?

Kaluza is licensed through the use of a hardware key, commonly known as a romlock or dongle. These keys provide easy portability of software from one computer to another and for analysis on your laptop when you’re not in the lab.

 

How does the network license work?

Network licenses are based on the number of concurrent sessions running Kaluza. An unlimited number of computers can have the software installed. The network license will control the maximum number of users are actively logged into the network key. The key management software can be easily access through a web-based program.

 

What are the recommended computer specifications?

Kaluza uses a patented technology that revolutionizes the speed at which data is analyzed. As with all software, speed is dependent on hardware specifications as well as the size and complexity of the data being handled. For ultimate performance we recommend the following computer specification.

Users may choose to purchase a system that has been configured and tested with Kaluza Software. System options include–
 

Tesla Workstation Models
Dell Dell Precision T7500
Lenovo S20 configuration with NVIDIA Tesla C1060 or C2050  
Non-Tesla Workstations  
Dell T5500 4GB dual CPU
Lenovo S20 4GB dual CPU configuration

 

How do I get more performance from my current system?

The Event Sampling Mode optimizes performance with large event files on sub-optimal computer workstations. Select from the Kaluza Options dialog from the main launch icon. This feature will help by temporarily decreasing the number of events that appear on the plot.

 

What operating systems are compatible with Kaluza?

Kaluza is compatible with Windows XP and Vista 32-bit PC environments as well as MAC hardware with operating systems under dual-boot programs such as "Bootcamp".  Kaluza 1.1 is compatible with Windows 7 (32bit/64bit operating systems).

OS X systems running in a virtual machine environment with Windows such as "Parallels" and "VirtualBox" has not been verified and has been known to show visual glitches. Also, the demo mode in virtual machines has been disabled.

 

What new features have been added to Kaluza 1.3? 

  • Batch analysis
  • Gate on radar plots
  • Geometric mean
  • Comparison plot
  • Gating hierarchy
  • Information plot with user-defined expressions
  • Export compensated data
  • New auto-compensation algorithms
  • Customizable data set labels
  • Change font on plots, titles, axes
  • High-contrast plot background color (black)
  • Higher plot resolution
  • User-configured options for Add All Plots
  • Separate gating spaces for each data set in a composite protocol to allow color precedence (event coloring) per data set
  • User-selected defaults in Kaluza Options

 

What language packages are available for Kaluza 1.3?

Kaluza is available also in Simplified Chinese and Japanese. Additional language packages will be posted as they become available.

 

Language pack downloads

Refer to the Documents section below.

 

I have Kaluza 1.0 or Kaluza v1.1. How do I get an upgrade to 1.3?

Contact a Beckman Coulter Customer Service Representative for your free upgrade.  Ask for PN A45967. Your existing hardware key will work with version 1.3.

Training Courses

File/Info Title Ref. Number
Kaluza Flow Cytometer Training Modules

Videos

  • Kaluza for Gallios - Using the Simulator with Modern Browser

    Click to play  

  • Kaluza for Gallios Compensation Tutorial - Creating a Compensation Matrix

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  • Kaluza for Gallios Compensation Tutorial - Creating a Compensation Worklist

    Click to play  

  • Kaluza for Gallios Compensation Tutorial - Using the Simulator with a Legacy Browser

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  • Kaluza Software: ASCB 2013 Kaluza Prize

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  • Kaluza Software: Experience the performance difference of Kaluza

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    Kaluza´s revolutionary speed makes analysis of multiple datasets as simple as drag & drop. Patented data processing technology enables simultaneous analysis of many large files.
  • Kaluza Software: Extended Features for Kaluza Analysis

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  • Kaluza Software: Getting to Know Your Kaluza Acquisition Software

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  • Kaluza Software: Intelligent design

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    Kaluza is designed to simply, efficiently and quickly analyze multicolor, multiparametric data from cytometers of today and tomorrow. Kaluza´s intelligent design provides an intuitive environment for data exploration.
  • Kaluza Software: Launch

    Click to play  

  • Kaluza Software: Parametric Visualization

    Click to play  

    Kaluza´s innovative multiparametric plots revolutionize the way multicolor data is analyzed. Multidimensional visualization tools can significantly reduce time required for complex data analysis.
  • Kaluza Software: Performance

    Click to play  

    Kaluza’s revolutionary speed makes analysis of multiple datasets as simple as drag & drop. Patented data processing technology enables simultaneous analysis of many large files.
  • Kaluza Software: You Have Your Data, Now What? Setting Up Statistics

    Click to play  

  • Kaluza Tutorial: Attributes Pane

    Click to play  

    The Kaluza Attributes pane displays the data file’s Parameters, Compensation, and Color Precedence details.

    Click on the Parameters tab to view the data file’s Parameter Names and Descriptions. Click on the chevron to Show...

  • Kaluza Tutorial: Creating Plots

    Click to play  

    Plots are easily added to a dataset by clicking on the desired plot type from the Plots and Tables Ribbon tab. To assign parameters to the plot, simply click on the parameter hyperlink on each axis. Any plot type may be dragged and dropped from...

  • Kaluza Tutorial: Opening data files

    Click to play  

    Data files may be opened in Kaluza by:

    Selecting the Open Files button in the center of the Kaluza workspace. Alternatively, you may open files from the Application Menu located at the upper left of the Kaluza workspace. Also from the...

  • Kaluza Tutorial: Overlay Plot

    Click to play  

    An overlay plot is a combination of multiple histogram plots displayed in a single plot. It is a highly visual data comparison tool and up to 8 histograms may be added for display. Several formatting tools are available through the Plot Radial...

  • Kaluza Tutorial: Radial Menus

    Click to play  

    Radial menus provide quick access to a variety of editing tools in Kaluza.  Simply right click on a plot, gate, Plot Sheet or Report sheet to view the Radial menus.  As you hover over any Radial menu icon, a menu appears allowing you...

  • Kaluza Tutorial: Tree Plots

    Click to play  

    When working with high color in flow cytometry, the number of data plots increases significantly with each color added to the analysis. A Tree Plot is a useful data comparison tool that can condense data from up to 28 bivariate plots. It is...

  • Webinars & Symposiums

  • Data Flow Rethinking Data Analysis for Flow Cytometry - Kaluza 1.1

    Sharlene Wright, Beckman Coulter

    Click to play  

    Designed to quickly and efficiently analyze multi-color, multi-parametric high content data generated by today's flow cytometers, the revolutionary Kaluza analysis software provides novel visualization tools, real time speed and powerful...
  • DuraClone - A New Frontier in Dry Reagents

    Aprajita Priyadarshini, Sr. Applications Scientist, Beckman Coulter

    Click to play  

    DuraClone is an innovative approach for dry reagents for flow cytometry. The proprietary process results in a product that is consistent from tube to tube and lot to lot and eliminates the need for cold chain storage. The process is distinct...

  • Microvesicle Detection and Cell Sorting

    Vasilis Toxavidis, MChS, HCPR Resource Director, Beth Israel Deaconess Medical Center Flow Cytometry Core Facility, Harvard Stem Cell Institute John Tigges, Beth Israel Deaconess Medical Center Flow

    Click to play  

    There is great interest in both medical and scientific communities in submicron cell derived particles, termed microparticles or microvesicles. A great difficulty in this field, however, has been the optimization and standardization of...

  • Stem Cells: Identification & Isolation through High-Speed Cell Sorting

    E. Michael Meyer, Research Scientist Manager, Flow Cytometry Facility University of Pittsburgh Cancer Institute

    Click to play  

    The discussion of Stem Cells can cover a range of topics and cell types; hematopoietic progenitor cells, mesenchymal, adipose, and even "cancer" stem cells are just few of the types of stem cells that we work with on a daily basis...

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