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Purification & Clean-Up

RNA & cDNA Clean Up

Agencourt RNAClean XP

A simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions.

 

Overview

Agencourt RNAClean XP with Scalable throughput
 

The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions.


Agencourt RNAClean XP Features
 
  • Purification of small and large nucleic acid products
  • Complete removal of salts, unincorporated primers and dNTPs
  • Simple automation-friendly protocol
  • No centrifugation, filtration or precipitation steps required
  • Elution in aqueous solution
  • Purifies both cDNA and cRNA
  • Scalable throughput  

Process Overview



1. Enzymatic reaction 2. Binding of cDNA to magnetic beads 3. Separation of total RNA bound to magnetic beads from contaminants 4. Washing of RNA with Ethanol 5. Elution of RNA from the magnetic particles. 6. Transfer away from the beads into a new plate.


High Yield

The Agencourt RNAClean system consistently recovers more cRNA than standard column-based cleanup methods. The kit is effective in two methods for producing cRNA for microarray analysis, IVT and NuGEN* Amplification Technology. As shown below (Figure 1), the Agencourt RNAClean XP kit produced a higher yield of RNA over the RNeasy* kit across both amplification methods.



Figure 1. In vitro transcribed cRNA products produced from the same biological sample were purified using the Agencourt RNAClean XP kit or the RNeasy column-based system. IVT target was PCR products that contained a T7 sequence on the forward primer. IVT was performed using an Ambion* MEGAscript* T7 Kit. NuGEN Target was total RNA from mouse liver extracted using the RNAdvance Tissue kit. Amplification was performed using the NuGEN RNA amplification system V2. Samples were pooled and diluted prior to extractions. Chip analysis was performed using RNA Nano Chip on the Agilent* Bioanalyzer.
 

Versatile and Economical

The Agencourt RNAClean XP kit is a fast and flexible solution for cleaning up enzymatic reactions. To accommodate various existing laboratory processes, the RNA purified using this chemistry can be run using a variety of IVT labeling kits. Even without the use of organic solvents, centrifugation, or vacuum filtration, RNA is purified in approximately 20 minutes.
 

Specifications
 
Part No. Description
 UNSPSC  41105518
 Volume  40 mL


‡ Phillips J., and Eberwine J.H. 1996. Antisense RNA Amplification: A Linear Amplification Method for Analyzing the mRNA Population from Single Living Cells. Methods 10: 283-288.
* All trademarks are property of their respective owners.

 

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