SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing. In this process, size selection is required to produce a uniform distribution of fragments around an average size. Using SPRIselect, the size distribution can be adjusted to suit the application and platform used. The process can be scaled for low to high throughput workflows.
The guidance provided below can be used to optimize the desired size selection range. Used manually or automated on a liquid handling system such as the Biomek Laboratory Automation Workstation, SPRIselect will provide rapid and consistent size selection suitable for most applications.
Bee Na Lee, PhD, Staff Application Scientist, Beckman Coulter
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SPRIselect Reagent 450mL
SPRIselect Reagent 5mL
SPRIselect Reagent 60mL
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