Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years. Challenges to researchers working with exosomes include setting up density gradients by hand, because it is tedious, time-consuming and subject to user, lab, and method variability. There also is a need for greater accuracy in size and concentration analysis. At the same time, experts in the field have called for the establishment of standard protocols.1 This paper focuses on solutions to those challenges through cost-effective, large-scale purification, and fast analysis of exosomes. Specifically, the Biomek 4000 Workstation helps overcome human variables and provides a consistent, reproducible, high-throughput method for gradient setup, representing a breakthrough solution to scale-up problems. Optima Ultracentrifugation Series helps researchers maintain reliability between runs, making outcomes highly reproducible. The DelsaMax CORE saves time and cost of TEM analysis for size and concentration.