Automating a Linear Density Gradient for Purification of a Protein
Proteins have a variety of cellular functions, structures, and mechanisms of action. Routinely, proteins bind other biomolecules, or ligands, in order to complete a task. Researchers gain valuable knowledge on how proteins work in a cellular environment by purification of proteins bound to their appropriate substrate. Typically, protein:ligand complexes are further analyzed by cryo-EM, NMR, and/or x-ray crystallography for structural information. However, purification of protein:ligand complexes remains challenging due to the lack of robust, reproducible separation techniques.