Micro RNAs are small, naturally occurring non-coding ribonucleic acids with sizes between 18 and 40 nucleotides (nts) that have been demonstrated to play a significant role in the regulation of gene expression. As a result, interest in smaller RNA species such as miRNA has increased. This application note describes the purification of miRNA and total RNA from fresh-frozen tissue samples using the Beckman Coulter SPRI (Solid Phase Reverse Immobilization) magnetic bead-based chemistry and the Biomek automated extraction method. The RNAdvance miRNA Tissue 96 demonstrated method enables automated purification of total RNA, including miRNA and other small RNAs, from 1 to 96 samples on a Biomek Span-8 Workstation. Total RNA and miRNA can be purified from very small amounts of animal tissue as starting materials. The Biomek automated SPRI method is an easy, high-yielding and robust nucleic acid purification process that does not require centrifugation and vacuum filtration steps. Purified nucleic acids are easily eluted from the magnetic beads under aqueous conditions, which provide maximum flexibility for downstream applications. The data shows that the samples extracted using the Biomek gave comparable RNA yield, miRNA and messenger RNA gene expression as compared to samples extracted manually.