The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification. Solid Phase Reversible Immobilization (SPRI) bead chemistry is utilized throughout the protocol for size selection and sample cleanup steps. The process can be laborious and error-proned and therefore is ideal for Biomek automation. Choose from low to high throughput Biomek liquid handling workstations to automate all steps of the workflow providing the option for a complete walk-away solution. The methods are proven to deliver sequence- ready libraries through demonstrated performance using scientifically relevant samples. These ready-to implement methods are compatible with either the LT or HT versions of the Illumina TruSeq DNA PCR-Free Sample Preparation kits (Illumina P/N FC-121-3001, FC-121-3002 or FC-121-3003).