Automated Illumina TruSeq Custom Amplicon Low Input Library Preparation Kit on the Beckman Coulter Biomek FXP Automated Liquid Handler.
The ability to rapidly sequence selected regions of a genome with high levels of coverage is essential for many applications and market segments ranging from the characterization of rare variants in FFPE cancer research to identification of genotype within large sample populations via Genotyping by Sequencing (GBS) in Agrigenomics. The Illumina TruSeq Custom Amplicon Low Input Library Preparation kit (FC-134-2001) allows for the characterization of up to 1536 amplicons spanning approximately 600kb in a single multiplex reaction for each sample. The kit supports input DNA amounts as low as 10 ng per sample and can be used with FFPE DNA samples depending on the quality of the sample. The kit is intended for use with customer designed panels developed using DesignStudio (http://www.illumina.com/informatics/research/experimental-design/designstudio.html).