Utilizing Automated Assay Optimization software for BioRAPTR to maximize antibody production by a hybridoma cell line
Cell culture consists of numerous processes that can benefit from optimization.One example is optimizing media conditions to maximize the production of a protein of interest. We used design of experiment (DOE) to generate an array of media component combinations to determine what conditions would produce the most IgG molecules from a hybridoma culture. Automated Assay Optimization (AAO) software from Beckman Coulter transformed the output from the statistical design software into dispensing volumes for use with the BioRAPTR FRD* microfluidic workstation. With a single experimental design, we increased the IgG titer by 45% over standard growth conditions and established serum-free cultures that produced >90% of the titer in standard culture conditions. These results illustrate the rapid and powerful returns that can be achieved through process optimization using AAO for BioRAPTR software.