Over the past few years, the scientific community has placed great importance on characterizing small RNA molecules, in particular the microRNAs (miRNA). miRNAs are ribonucleic acids that range from 18 nucleotides to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of miRNA. In order to measure miRNA more precisely and achieve better quality miRNA sequencing data, it is desirable to remove the larger unwanted ribosomal and other species of RNAs. This technical note describes the use of Beckman Coulter‘s SPRIselect reagent kit for miRNA enrichment. The Solid Phase Reverse Immobilization (SPRI) magnetic bead method is a nucleic acid purification procedure that does not require centrifugation or filtration steps. This simple, rapid, automation friendly and produces high quality samples.