Lentiviral Vector Preparation
Viral vectors are one of the most commonly used tools for genetic manipulation in mammalian cells. They are used both for gene expression as well as gene silencing. Lentiviruses are commonly used viral vectors due to their ability to infect both dividing and non-dividing cells, including stem cells. When infecting cells with these viral vectors, it is often necessary to concentrate virus particles to achieve high titer, especially when a large number of cells must be infected or for use with certain cell lines which are resistant to transduction. Centrifugation is a simple and effective method to concentrate viral particles. Here we present two simple methods of concentrating the viral vector. These methods are equally useful for concentrating viral particles for other studies, like nucleic acid sample prep or electron microscopy.