Rapid-Determination of Spectrophotometric Absorptivity by AUC
Spectrophotometric determination of protein concentrations is an important analytical tool for nearly all phases of protein drug development and manufacturing. Determinations of absorptivities (A0.1%) and molar extinction coefficients (ε) for protein solutions require at least one initial, accurate measurement of protein concentration. Traditionally, this crucial concentration has been determined by dry weight analysis, total nitrogen analysis, or quantitative amino acid analysis. Unfortunately, these techniques are labor intensive, may require relatively large sample size and can give unreliable results.