To the Standardization of Exosome Isolation and Characterization
Exosomes are small vesicles, most often described in the literature to be less than 150 nm and have been demonstrated to be released by a wide variety of cell types, exhibiting a multitude of functions, and proven to be involved in cancer metastasis. Standardization of isolation and characterization methods is critical to the advancement of this exciting, emerging field. Density gradient ultracentrifugation is frequently the preferred choice for exosome isolation, generating highly pure sample preparations; however, the workflow often lacks reproducibility among laboratories and users. Next generation small RNA sequencing is one of many downstream assays for exosome characterization and biomarker identification, but again the protocols employed often vary drastically. Here we present a solution using the Biomek 4000 Genomic Workstation to increase throughput, walk-away time, reproducibility, and accuracy of results.
Indianapolis, IN, 46268