The Agencourt® RNAdvance® Cell v2 kit utilizes Agencourt’s patented SPRI® paramagnetic bead-based technology and is specially designed to isolate total RNA from 200-50,000 cultured eukaryotic cell lines. The total RNA extraction procedure was modified for micro-RNA (miRNA) purification by optimizing the formulation of the Bind Buffer, Wash Buffer and ethanol wash steps. This supplemental protocol enables recovery of miRNA, small RNA and total RNA, for applications such as RT-PCR. The protocol can be performed in a 96-well plate or in a 1.7 mL tube format. The Agencourt® RNAdvance® Cell v2 procedure does not require vacuum filtration or centrifugation.