MicroRNAs (miRNAs) are small non-coding ribonucleic acids (~22 nt) that play important roles in gene regulation by binding to and repressing the activity of specific target mRNAs. It is known that tumor cells release miRNAs and RNA into the blood circulation. Profiles of miRNAs and RNA in plasma and serum have been found to be altered by cancer and other disease states. Therefore, discovery of cell-free circulating miRNAs and RNA biomarkers in the bloodstream or body fluids provides for the possibility of non-invasive diagnosis in early stages.
This application note describes the purification of miRNA and RNA from plasma and serum using Beckman Coulter’s Agencourt RNAdvance Blood kit. Beckman Coulter’s SPRI (Solid Phase Reverse Immobilization) paramagnetic beadbased chemistry provides an easy, rapid, high yielding, robust and automation-friendly nucleic acid purification procedure that does not require vortexing, centrifugation or filtration steps. The data shows that same protocol can be used for miRNA and RNA extraction from plasma and serum. The spiked in miR39 control recovery is comparable to a commonly used column kit.