Phytoplankton conversion of light on the upper limits of the ocean consists of half of the photosynthesis on the Earth. Population densities indicate health of phytoplankton, and the entire aquatic ecosystem. The isolation and sorting of aquatic samples using flow cytometry allows for quick and effective population analysis. Forward scatter on the Astrios EQ allows for differentiation of small and large particles from 0.2 to 30 μm on FSC. This design provides researchers greater flexibility to isolate and sort specific phytoplankton of different sizes while utilizing the 7 laser, 42 parameter instrument.
Photosynthetic plankton is one sorting application for the Astrios EQ forward scatter design. Marine Biology has used flow cytometry with phytoplankton previously for: rapid and objective evaluation of water quality1; to analyze grazing2; cell viability3; rapid strain identification4; diversity assessment5; and to characterize plankton populations through molecular6,7 and genetic approaches8.
Plant Pigments Absorption Spectra In a general photosystem, as seen in Figure 1, the chlorophyll b transfers energy through the antenna pigment complex to chlorophyll in a reaction center. The accessory pigments—such as Phycocyanin, Allophycocyanin, Allophycocyanin B, and Phycoerythrin—provide a composite absorption spectrum such that a wider range of visible and infrared radiation is absorbed by plants and algae.