A Flexible Solution for Automating Cell Staining Using the Biomek 4000 Workstation
The automated Cell Staining Application on the Biomek 4000 Workstation can improve throughput and reproducibility of conventional staining of live and fixed adherent cells. This application provides a walk-away solution for staining cells in a variety of workflows, including antibody staining, nuclear dyes, and viability assays in a 96-well plate. Flexibility is central to this application, allowing users to choose and customize any combination of steps (fixation, permeabilization, washing, blocking, and staining) by configuring options in a simple User Interface. Manual staining of cells, either to characterize antigen expression or to assess cell health, is labor-intensive and timeconsuming. Here we demonstrate several automation strategies using the Cell Staining Application on the Biomek 4000 Workstation.