Chinese Hamster Ovary (CHO) Cell Analysis Using the Beckman Coulter Vi-CELL™
Chinese Hamster Ovary Cells (CHO) are among the most widely used mammalian cells for the expression of a wide variety of recombinant proteins. The cells are routinely cultured on a large scale in bioreactors. The heterogeneous protein product produced is further refined downstream and ultimately used for pharmaceutical purposes. In order to determine the health of these cell cultures, both cell concentration and percentage viability are measured over time. For percentage viability, the Vi-CELL automates the labor-intensive hemacytometer method using Trypan Blue dye.