Cellular applications require consistent plating of cells and sample preparation so that cellular changes can be accurately attributed to experimental treatments. Confirmation of consistent plating can be achieved through imaging wells or through surrogate assays such as XTT cell viability kits. These assays can also be used to detect differences in cell growth, such as in cell toxicity or media optimization screens. Automation can improve the consistency of cell plating and assay sample preparation and reduce the opportunity for errors. Here we show how the Biomek i5 Workstation (Figure 1) was used to serially dilute a cell suspension, plate the resulting cells and prepare them for analysis by XTT.