To streamline the NGS sample preparation process, Beckman Coulter has recently launched the SPRIselect Reagent Kit to allow NGS sample size selection to be automated on Biomek liquid handling platforms for high throughput and high efficiency sample prepar ations. The data presented here demonstrates the automation of the SPRIselect Kit on the Biomek 4000 Workstation. The demonstrated method will prepare 96 samples in 96-well plate format for small to medium throughput laboratories.
SPRIselect is a SPRI-based (Solid Phase Rev ersible Immobilization) par amagnetic bead technology. To demonstrate the capabilities of SPRIselect, three different shears of E. Coli gDNA ranging between 100 – 2000bp were pooled into a “Mega Shear” as the ratio (bead to sample volume) is altered. Our data demonstrates successful selections using 0.65x ratio for Left Side Selection, 0.7x ratio for Right Side Selection, and three ranges of Double Side Selections (0.9 L-0.5 R; 0.85 L-0.56 R; and 0.8 L – 0.61 R) on the Biomek 4000 platform.