Glycerol is an important platform chemical and also serves as feedstock for a broad range of
bioprocesses. Yeast strains are known to produce glycerol under certain conditions. We intended to identify yeast strains with improved capacity to produce glycerol from renewable feedstocks. As even small improvements in glycerol formation have a strong commercial impact, we took special care of precise and reliable methods for glycerol quantification. Due to the high number of yeast strains to be tested (> 10000), standard HPLC-methods were ruled out for routine analysis due to time constraints. Colorimetric detection of glycerol by a coupled enzymatic assay is highly sensitive and can be scaled in numbers to meet demands. Automation of the workflows was mandatory, and we employed the Biomek 4000 Laboratory Automation Workstation. A
commercial enzymatic kit for the detection of glycerol in complex biological samples was implemented for our screening. We demonstrate successful scale-down of the standard assay from the ml to the μl-scale, thus
enabling screening in 96-well microplates.