Automation of RNA isolation from blood samples stabilized in RNAgard® Blood tubes, using the magnetic bead-based Agencourt RNAdvance Blood system
Clinical Research studies often require blood sample collection at multiple geographic sites under a wide range of conditions. RNAgard® Blood Tubes are designed for the immediate stabilization of cellular RNA in human blood samples, providing an efficient method for standardized collection, transport and storage of whole blood specimens and isolation of their RNA material. Purification of RNA from blood stabilized in RNAgard Blood Tubes has been optimized with the BioMaxi Blood RNA Purification Kit (Biomatrica). High yields of high quality RNA with unaltered gene expression are obtained, and perform well in a wide range of downstream research applications, including but not limited to, bioanalyzer, gene quantification by qPCR and gene expression arrays. In order to accommodate the need for high throughput processing of samples, here we present an easy workflow for automated RNA isolation from blood samples collected and stored in RNAgard blood tubes, using the magnetic bead-based Agencourt® RNAdvance™ Blood it (Cat# A35603 Beckman Coulter, Inc). We show that using this RNA isolation platform, high yields of great quality RNA can be isolated from blood stabilized in RNAgard Blood tubes, even after 2 weeks of ambient temperature storage. The combination of the great RNA stabilization provided by Biomatrica’s RNAgard Blood Tubes and the high throughput capacity of Agencourt’s RNAdvance Blood Kit provides an excellent solution for ambient temperature blood sample collection, storage and RNA isolation for very high sample number processing requirements.