The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples. Solid Phase Reversible Immobilization (SPRI) bead chemistry is utilized throughout the protocol for size selection and sample cleanup steps. The process can be laborious and error-prone and is therefore ideal for Biomek automation. Choose from low to high throughput Biomek liquid handling workstations to automate all steps of the workflow providing the option for a complete walk-away solution. The methods are proven to deliver sequence- ready libraries through demonstrated performance using scientifically relevant samples. These ready-to-implement methods are compatible with Illumina TruSeq Exome Library Preparation kits (Illumina P/N FC-150-001, FC-150-002 or FC-150-003).