Extracellular miRNAs that circulate in the bloodstream, known as circulating miRNAs, are remarkably stable. These small non-coding ribonucleic acids (~22 nt) play important roles in gene regulation by binding to and repressing the activity of specific target mRNAs. It is known that tumor cells release miRNAs and RNA into the blood circulation. Profiles of miRNAs and RNA in plasma and serum have been found to be altered in cancer and other disease states. Therefore, circulating miRNAs have been proposed as disease biomarkers that may aid in risk assessment, diagnosis, prognosis, and monitoring of treatment response. This application note describes the purification of miRNA and total RNA from 200μL of serum/plasma samples using the Beckman Coulter SPRI (Solid Phase Reverse Immobilization) magnetic bead based chemistry and a Biomek automated extraction method. The RNAdvance miRNA Blood 96 demonstrated method enables automated purification of total RNA, including miRNA and other small RNAs, from 8–96 samples on a Biomek Span-8 automated workstation. The Biomek automated SPRI method is an easy, high yielding and robust nucleic acid purification process that does not require centrifugation and vacuum filtration steps. Purified nucleic acids are easily eluted from the magnetic beads under aqueous conditions, which provides maximum flexibility for downstream applications. The data shows that Biomek automated extraction yielded in consistent miRNA recovery with low CV% using control miRNA samples. The Biomek demonstrated method successfully extracted miRNAs and messenger RNA with yields comparable to manually extracted samples.