Sorting: Enhanced Forward Scatter Module+ on the MoFlo™ Astrios*: Small Particle Applications
The MoFlo™ Astrios instrument from Beckman Coulter is a high-speed cell sorter capable of 6-way sorting speeds up to 70,000 events per second with 99% purity. Improvements to this high end instrument have been made which allow scientists to identify particles as small as 0.2 μm using Forward Scatter trigger. Specifically, these improvements to forward scatter have enabled scientists to take advantage of the following cell biology applications: S. cerevisiae, E. coli, murine mitochondria and photosynthetic plankton such as E. huxleyi, and P. marinus. S. cerevisiae and E. coli are common organisms used in the field of cell biology. Isolation of mitochondria for use in DNA analysis as well as focused research on a subcellular component is of great interest to researchers. E. huxleyi and P. marinus are highly-characterized organisms studied in marine biology and have been suggested to play critical roles in the carbon cycle. The improvements to Forward Scatter on the MoFlo™ Astrios instrument now allow scientists to further refine their work in these popular fields of research.