a-Chymotrypsin as a Model System to Demonstrate the Use of Data Simulator in the New Beckman XL-A/XL-I Data Analysis Software
The Optima XL-A/XL-I Data Analysis Software from Beckman includes a program in the Utilities section that synthesizes equilibrium data according to parameters set by the user. This article will describe and define the parameters available to the user and the graphics associated with the software. A simple monomer-dimer reversible self-associating system will be used as a model to compare synthesized data with data collected from the analytical ultracen-trifuge. This comparison will show the user how to take advantage of the simulator to design optimal experimental conditions such as initial protein concentrations and rotor speeds according to the characteristics of the association process. Chymotrypsin at pH 4.0 was chosen for this system since it has been well characterized in the literature(1) and repeat analysis shows consistent results with the reported association constants.(2) The simulator will be used to generate data with various contaminants or amounts of monomer without the ability to self associate (also known as incompetent monomer) to demonstrate the differences in the simulated data from the “ideal” system. Also, it will be apparent how the simulator can be used to set up better experimental conditions for accuracy in modeling data.
Technical Application Information