Automated FFPE DNA Extraction Using the FormaPure DNA Extraction Kit using the Biomek series of automated liquid handlers from Beckman Coulter, Inc.
Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential for many cancer research applications. The FormaPure DNA extraction kit from Beckman Coulter, Inc. provides a convenient extraction process utilizing Beckman Coulter proprietary solid phase reversible immobilization (SPRI) chemistry to extract DNA from FFPE curls. Briefly, FFPE curls are deparaffinized, after which a lysis buffer is added in conjunction with Proteinase K to lyse the tissue. Following tissue digestion, the DNA is decrosslinked and the lysate is transferred to a new plate. RNase A is then used to digest unwanted RNA from the sample, after which the remaining DNA is bound to magnetic beads using a proprietary binding buffer. Following binding, the beads containing the DNA are washed to remove unwanted nucleotides and other contaminants, after which the DNA is eluted from the beads.