The Agencourt RNAdvance Tissue total RNA purification kit utilizes Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology to isolate micro RNA (miRNA) and total RNA. The protocol can be performed in both 96-well and single tube formats. Purification begins with the homogenization and lysis of tissue. Following lysis, there is an immobilization of RNA onto the magnetic beads allowing for the RNA to be separated away from contaminants using a magnetic field. The RNA is then treated with DNase and the contaminants rinsed away using a simple wash procedure. The Agencourt RNAdvance Tissue kit is amenable to automation as it utilizes magnetic separation, thus eliminating the need for vacuum filtration or centrifugation.