Here we describe immunophenotypic analysis of human peripheral blood lymphocytes utilizing a 6-color antibody panel in a stain/lyse protocol run on the CytoFLEX* Research Flow Cytometer. The markers choosen are commonly used surface CD antigens which can be used to quantify and characterize normal and abnormal lymphocyte populations in peripheral blood.
The CytoFLEX is excellent for immunophenotyping research experiments. The proprietary Wavelength Division Multiplexing (WDM) detection module uses solid-state, high efficiency, low-noise Fiber Array Photodiode Detectors (FAPD), giving exceptional resolution for more precise data and better detection of rare events. This protocol describes the use of the CytoFLEX with a red and blue laser configuration for six-color immunophenotyping analysis.